Sleep.
It's something we spend about a third of our lives doing,
but do any of us really understand what it's all about?
Two thousand years ago, Galen,
one of the most prominent medical researchers
of the ancient world,
proposed that while we're awake,
our brain's motive force, its juice,
would flow out to all the other parts of the body,
animating them but leaving the brain all dried up,
and he thought that when we sleep,
all this moisture that filled the rest of the body
would come rushing back,
rehydrating the brain
and refreshing the mind.
Now, that sounds completely ridiculous to us now,
but Galen was simply trying to explain
something about sleep
that we all deal with every day.
See, we all know based on our own experience
that when you sleep, it clears your mind,
and when you don't sleep,
it leaves your mind murky.
But while we know a great deal more about sleep now
than when Galen was around,
we still haven't understood why it is that sleep,
of all of our activities, has this incredible
restorative function for the mind.
So today I want to tell you about
some recent research
that may shed new light on this question.
We've found that sleep may actually be
a kind of elegant design solution
to some of the brain's most basic needs,
a unique way that the brain
meets the high demands and the narrow margins
that set it apart from all the other organs of the body.
So almost all the biology that we observe
can be thought of as a series of problems
and their corresponding solutions,
and the first problem that every organ must solve
is a continuous supply of nutrients to fuel
all those cells of the body.
In the brain, that is especially critical;
its intense electrical activity uses up
a quarter of the body's entire energy supply,
even though the brain accounts
for only about two percent of the body's mass.
So the circulatory system
solves the nutrient delivery problem
by sending blood vessels to supply nutrients
and oxygen to every corner of our body.
You can actually see it in this video here.
Here, we're imaging blood vessels
in the brain of a living mouse.
The blood vessels form a complex network
that fills the entire brain volume.
They start at the surface of the brain,
and then they dive down into the tissue itself,
and as they spread out, they supply nutrients
and oxygen to each and every cell in the brain.
Now, just as every cell requires
nutrients to fuel it,
every cell also produces waste as a byproduct,
and the clearance of that waste
is the second basic problem
that each organ has to solve.
This diagram shows the body's lymphatic system,
which has evolved to meet this need.
It's a second parallel network of vessels
that extends throughout the body.
It takes up proteins and other waste
from the spaces between the cells,
it collects them, and then dumps them into the blood
so they can be disposed of.
But if you look really closely at this diagram,
you'll see something
that doesn't make a lot of sense.
So if we were to zoom into this guy's head,
one of the things that you would see there
is that there are no lymphatic vessels in the brain.
But that doesn't make a lot of sense, does it?
I mean, the brain is this intensely active organ
that produces a correspondingly large amount of waste
that must be efficiently cleared.
And yet, it lacks lymphatic vessels, which means that
the approach that the rest of the body takes
to clearing away its waste
won't work in the brain.
So how, then, does the brain solve
its waste clearance problem?
Well, that seemingly mundane question
is where our group first jumped into this story,
and what we found
as we dove down into the brain,
down among the neurons and the blood vessels,
was that the brain's solution
to the problem of waste clearance,
it was really unexpected.
It was ingenious,
but it was also beautiful.
Let me tell you about what we found.
So the brain has this large pool
of clean, clear fluid called cerebrospinal fluid.
We call it the CSF.
The CSF fills the space that surrounds the brain,
and wastes from inside the brain
make their way out to the CSF,
which gets dumped, along with the waste, into the blood.
So in that way, it sounds a lot like
the lymphatic system, doesn't it?
But what's interesting is that the fluid and the waste
from inside the brain,
they don't just percolate their way randomly
out to these pools of CSF.
Instead, there is a specialized network of plumbing
that organizes and facilitates this process.
You can see that in these videos.
Here, we're again imaging into the brain
of living mice.
The frame on your left shows
what's happening at the brain's surface,
and the frame on your right shows
what's happening down below the surface of the brain
within the tissue itself.
We've labeled the blood vessels in red,
and the CSF that's surrounding the brain
will be in green.
Now, what was surprising to us
was that the fluid on the outside of the brain,
it didn't stay on the outside.
Instead, the CSF was pumped back into
and through the brain
along the outsides of the blood vessels,
and as it flushed down into the brain
along the outsides of these vessels,
it was actually helping to clear away,
to clean the waste from the spaces
between the brain's cells.
If you think about it,
using the outsides of these blood vessels like this
is a really clever design solution,
because the brain is enclosed
in a rigid skull
and it's packed full of cells,
so there is no extra space inside it
for a whole second set of vessels like the lymphatic system.
Yet the blood vessels,
they extend from the surface of the brain
down to reach every single cell in the brain,
which means that fluid
that's traveling along the outsides of these vessels
can gain easy access to the entire brain's volume,
so it's actually this really clever way
to repurpose one set of vessels, the blood vessels,
to take over and replace the function
of a second set of vessels, the lymphatic vessels,
to make it so you don't need them.
And what's amazing is that no other organ
takes quite this approach
to clearing away the waste from between its cells.
This is a solution that is entirely unique to the brain.
But our most surprising finding
was that all of this,
everything I just told you about,
with all this fluid rushing through the brain,
it's only happening in the sleeping brain.
Here, the video on the left
shows how much of the CSF is moving
through the brain of a living mouse while it's awake.
It's almost nothing.
Yet in the same animal,
if we wait just a little while until it's gone to sleep,
what we see is that the CSF
is rushing through the brain,
and we discovered that at the same time
when the brain goes to sleep,
the brain cells themselves seem to shrink,
opening up spaces in between them,
allowing fluid to rush through
and allowing waste to be cleared out.
So it seems that Galen may actually have been
sort of on the right track when he wrote about
fluid rushing through the brain
when sleep came on.
Our own research, now it's 2,000 years later,
suggests that what's happening is that
when the brain is awake
and is at its most busy,
it puts off clearing away the waste
from the spaces between its cells until later,
and then, when it goes to sleep
and doesn't have to be as busy,
it shifts into a kind of cleaning mode
to clear away the waste
from the spaces between its cells,
the waste that's accumulated throughout the day.
So it's actually a little bit like how you or I,
we put off our household chores during the work week
when we don't have time to get to it,
and then we play catch up on all the cleaning that we have to do
when the weekend rolls around.
Now, I've just talked a lot about waste clearance,
but I haven't been very specific
about the kinds of waste
that the brain needs to be clearing
during sleep in order to stay healthy.
The waste product that these recent studies
focused most on is amyloid-beta,
which is a protein that's made in the brain all the time.
My brain's making amyloid-beta right now,
and so is yours.
But in patients with Alzheimer's disease,
amyloid-beta builds up and aggregates
in the spaces between the brain's cells,
instead of being cleared away like it's supposed to be,
and it's this buildup of amyloid-beta
that's thought to be one of the key steps
in the development of that terrible disease.
So we measured how fast amyloid-beta is cleared
from the brain when it's awake
versus when it's asleep,
and we found that indeed,
the clearance of amyloid-beta
is much more rapid from the sleeping brain.
So if sleep, then,
is part of the brain's solution
to the problem of waste clearance,
then this may dramatically change how we think
about the relationship between sleep,
amyloid-beta, and Alzheimer's disease.
A series of recent clinical studies
suggest that among patients
who haven't yet developed Alzheimer's disease,
worsening sleep quality and sleep duration
are associated with a greater amount
of amyloid-beta building up in the brain,
and while it's important to point out
that these studies don't prove
that lack of sleep or poor sleep
cause Alzheimer's disease,
they do suggest that the failure of the brain
to keep its house clean
by clearing away waste like amyloid-beta
may contribute to the development
of conditions like Alzheimer's.
So what this new research tells us, then,
is that the one thing that all of you
already knew about sleep,
that even Galen understood about sleep,
that it refreshes and clears the mind,
may actually be a big part
of what sleep is all about.
See, you and I, we go to sleep
every single night,
but our brains, they never rest.
While our body is still
and our mind is off walking in dreams somewhere,
the elegant machinery of the brain
is quietly hard at work
cleaning and maintaining
this unimaginably complex machine.
Like our housework,
it's a dirty and a thankless job,
but it's also important.
In your house, if you stop cleaning your kitchen
for a month,
your home will become completely unlivable
very quickly.
But in the brain, the consequences
of falling behind may be much greater
than the embarrassment of dirty countertops,
because when it comes to cleaning the brain,
it is the very health and function
of the mind and the body that's at stake,
which is why understanding these
very basic housekeeping functions of the brain today
may be critical for preventing and treating
diseases of the mind tomorrow.
Thank you.
(Applause)