大家好，欢迎回到synthetic biology 1 hi everybody welcome back to synthetic
毕加索（Pablo Picasso）说， biology 1 Pablo Picasso said that the
艺术家是情感的容器 artist is a receptacle for emotions that
这些情感来自任何地方，来自天空 come from anywhere from the sky from the
来自地球，来自一小块纸上 earth from a scrap of paper from a
通过转瞬即逝的形态或来自一张蜘蛛网 passing shape or from a spider's web in
这个项目我们使用转基因 this project we use genetically modified
细菌创造艺术，我们的灵感 bacteria to create art our inspiration
将是色蛋白，小的简单的 will be the chroma proteins small simple
色素鲜艳的蛋白质可以 brightly pigmented proteins that can be
在彩虹的每种颜色中都可以找到 found in every color of the rainbow
大自然已经在遍布地球的不同物种中 Nature has produced these proteins in
生产了这些蛋白质 different species all over the planet
合成生物学家收集它们 and synthetic biologists have collected
在某些情况下修改了它们 them modified them in some cases and
用实验室细菌表达它们，如果 express them in laboratory bacteria if
你是一个艺术灵魂，你可能会 you're an artistic soul you might be
受到一个有生命的颜料的创造力的启发 inspired by the creative possibilities
或者如果你不是一个 of a living paint or if you're not an
艺术灵魂，你可以专注于 artistic soul you can focus on the very
我们使用这些蛋白的实际原因是 practical reasons we have for using
它们很容易被追踪 these proteins they're easy to keep
因为与大多数游离蛋白不同 track of because unlike most free
我们只要看它们就能 proteins we can just see them we know
通过蛋白质的颜色知道我们改变了什么基因 what gene we transformed by the color of
我们通常 the protein that we make normally we
我们不需要DNA带来的奢华 don't have that luxury with DNA we need
我们使用特殊技巧使DNA可视化 to use special tricks to visualize DNA
并确定特定的诱导序列 and to identify specific sequences we
这个项目将介绍其中一些方法 will introduce some of those methods
色度蛋白将会让我们 in this project chroma proteins will get
考虑蛋白质浓度 us thinking about protein concentrations
以及如何控制它们 and how to control them are these cells
这些细胞是红色还是正红色 red or are they really red we'll take a
我们将仔细观察基因的各个部分 closer look at the parts of a gene that
这些基因控制蛋白质表达 control protein expression for the first
首先我们将特定DNA序列的变化 time we connect specific DNA sequence
跟特定结果联系起来 changes with specific outcomes in this
在这些案例中就是细胞颜色 case cell colors these are the places
在这里我们可以像DNA设计师一样 where we can make edits in the sequence
编辑序列，您想要天蓝色 as DNA designers so do you want sky blue
或宝蓝色 or royal blue
最后，我们将继续提升我们的 finally we'll continue to develop our
基础微生物实验室技能 basic microbiology lab skills I may not
我不是毕加索（Pablo Picasso），但我可以 be Pablo Picasso but I can streak out
在培养皿上培养漂亮的细胞 cells beautifully on a petri dish so
下回见，艺术就是生命，而生命才是 until next time art is life and life is
艺术 art