hey guys we're going to do a quick one 大家好，我们快来
hey guys we're going to do a quick one 位深度并将16位转换为
hey guys we're going to do a quick one on bit-depth and converting a 16-bit to 一个8位以节省大小和通话
on bit-depth and converting a 16-bit to 关于我们如何将其转换为
on bit-depth and converting a 16-bit to an 8-bit to save it on size and talk 什么是微妙之处，所以这是一个
an 8-bit to save it on size and talk 16位图像，它是16位文件
an 8-bit to save it on size and talk about how we go back converting that and 类型，我们将其转换为
about how we go back converting that and 这个日期实际上是8位五倍
about how we go back converting that and what the subtleties are so this one is a 在12位显微镜上拍摄的
what the subtleties are so this one is a 给我们带来了我们从这里得到的图像
what the subtleties are so this one is a 16-bit image and it's the 16-bit file 公爵杜克的核心手臂只是为了展示
16-bit image and it's the 16-bit file 您获得的不同的通用位范围
16-bit image and it's the 16-bit file type we're going to convert it down to 显微镜和照相机8位和12
type we're going to convert it down to 位在显微镜上更常见8位
type we're going to convert it down to an 8-bit five-time this date is actually 当你有东西的时候很棒
an 8-bit five-time this date is actually 像海妮，因为你实际上可以
an 8-bit five-time this date is actually taken on a 12 bit microscope so this 如果您使用的是PMT，则得到零值
taken on a 12 bit microscope so this 通常您想使用丰满度
taken on a 12 bit microscope so this brings us an image we've got here from a 12位4零九五
brings us an image we've got here from a 相机通常有65,000，我们有15
brings us an image we've got here from a Duke Duke core arm just to show the MX上的位相机
Duke Duke core arm just to show the 只有32个，但实际上4000个就足够了
Duke Duke core arm just to show the different common bit ranges you get on 255就足够了
different common bit ranges you get on 设置错误，它的宽容度较小
different common bit ranges you get on microscopes and cameras an 8-bit and 12 你可以有更多的娱乐空间
microscopes and cameras an 8-bit and 12 4000不太正确
microscopes and cameras an 8-bit and 12 bit are more common on microscopes 8-bit 所以这是一张12位的图像
bit are more common on microscopes 8-bit 一台计算机仅以8位存储，并且
bit are more common on microscopes 8-bit it's great when you've got something 16位使一切翻倍
it's great when you've got something 再加倍，所以没有12
it's great when you've got something like a heinie because you can actually 有点持有人
like a heinie because you can actually 因此它存储的是8位文件或
like a heinie because you can actually get a zero value if you're using a PMT 16位文件，所以16位文件是什么
get a zero value if you're using a PMT 我们在这里，但数据在此
get a zero value if you're using a PMT generally you want to use a fullness a 图像实际上最多只能达到4,000
generally you want to use a fullness a 4,000 ish让我们来看看
generally you want to use a fullness a 12 bit the 4 zero-nine-five 这些通道之一的直方图何时
12 bit the 4 zero-nine-five 在这里提供两对一频道，我们
12 bit the 4 zero-nine-five camera has often got 65,000 we have a 15 去日志只是为了看到绿色通道和
camera has often got 65,000 we have a 15 我在这里写了一些像素
camera has often got 65,000 we have a 15 bit camera on our MX which brings it 最大垂直排列是
bit camera on our MX which brings it 侧面指示器饱和，您可以
bit camera on our MX which brings it only to 32 but really a 4000 is plenty 看到有时候它会上升一个更高的是
only to 32 but really a 4000 is plenty 太饱和了，那很好，我们不要
only to 32 but really a 4000 is plenty 255 is enough but if you get the 我们希望这个直方图可以下降到
255 is enough but if you get the 这个角落，所以它不会走得太远
255 is enough but if you get the settings wrong it's less forgiving so 在那个角落，我们再来一个
settings wrong it's less forgiving so 蓝色通道的直方图
settings wrong it's less forgiving so you can have more room to play and kind 总是看着日志中的直方图
you can have more room to play and kind 看到它并没有占据整个范围，所以
you can have more room to play and kind of not quite get it right with the 4000 蓝色信号要少很多，所以我们
of not quite get it right with the 4000 可以将其从16位转换为
of not quite get it right with the 4000 so this is a 12 bit image that you're on 8位
so this is a 12 bit image that you're on 大多不用担心
so this is a 12 bit image that you're on a computer is only stored in 8 bits and 只是工作，重要的是
a computer is only stored in 8 bits and 您正在处理某些应用程序
a computer is only stored in 8 bits and 16 bits everything's doubled and doubled 更喜欢8位和其他时间
16 bits everything's doubled and doubled 有非常大的文件和Z堆栈，
16 bits everything's doubled and doubled doubled again and so there isn't a 12 缝合在一起确实有帮助
doubled again and so there isn't a 12 使它们变小
doubled again and so there isn't a 12 bit kind of holder 到8位，尤其是当您带上它们时
bit kind of holder 进入火星等3D软件
bit kind of holder so it's either stored an 8 bit file or a 像这样的东西
so it's either stored an 8 bit file or a 它可以帮助两个因素
so it's either stored an 8 bit file or a 16 bit file so the 16 bit file is what 确实有所作为，尤其是一次
16 bit file so the 16 bit file is what 你去三个维度，所以
16 bit file so the 16 bit file is what we have here but the data are in this 基本上，您要做的就是输入
we have here but the data are in this 然后将其从16更改为
we have here but the data are in this image actually only goes up to 4,000 8位之前，你必须
image actually only goes up to 4,000 意识到选项中的一种设置
image actually only goes up to 4,000 4,000 ish let's take a look at a 需要进行的转换
4,000 ish let's take a look at a 默认情况下已检查
4,000 ish let's take a look at a histogram one of these channels when to 玩早一点，但是你
histogram one of these channels when to 转换时必须有规模
histogram one of these channels when to offer the two to one channel here and we 如果不选择的话
offer the two to one channel here and we 将绝对数字转换为相同
offer the two to one channel here and we go log just to see the green channel and 绝对数字，所以它将被砍掉
go log just to see the green channel and 顶部并保留所有来自
go log just to see the green channel and we have a few pixels here that I write 0到5 5，以上所有内容
we have a few pixels here that I write 饱和到2到5，所以很混乱
we have a few pixels here that I write as a maximum the vertical line up is 真的，我确实有些震惊
as a maximum the vertical line up is 我认为不适合的情况
as a maximum the vertical line up is side indicator saturation and you can 这种情况下，请确保检查
side indicator saturation and you can 默认，但如果事情不顺利
side indicator saturation and you can see sometimes it goes up a higher one is 计划检查情况是否如此
see sometimes it goes up a higher one is 因此，我们还需要查看
see sometimes it goes up a higher one is too saturated and that's good we don't 对比必须卖的方式
too saturated and that's good we don't 要确定它如何改变
too saturated and that's good we don't we want this histogram to come down to 不同的价值体系
we want this histogram to come down to 很少，这是我们的字节数和对比
we want this histogram to come down to this corner so it doesn't go too far 并注意到它给了我
this corner so it doesn't go too far 该图像的直方图，这就是为什么
this corner so it doesn't go too far over that corner let's have another 变得奇怪，您必须单击
over that corner let's have another 窗口，然后单击回到此
over that corner let's have another histogram from the blue channel here and 项目控制在这里更新这是
histogram from the blue channel here and 告诉我最小和最大
histogram from the blue channel here and always look at the histogram in log you 在窗户上，那是我打的时候
always look at the histogram in log you 重置它将自动缩放
always look at the histogram in log you see it's not taking entire range and so 到窗口中的最小最大值
see it's not taking entire range and so 这个通道到绿色
see it's not taking entire range and so it's a lot lot less blue signal so we're 频道，您可以看到最低
it's a lot lot less blue signal so we're 最大整个12位范围，您
it's a lot lot less blue signal so we're going to can convert this from 16-bit to 可以看到他们Instagram表示在这里
going to can convert this from 16-bit to 然后在最小最大值之前
going to can convert this from 16-bit to 8-bit 如果蓝色通道达到1400，
8-bit 您使用自动按钮，它将
8-bit mostly don't need to worry about that 自动饱和
mostly don't need to worry about that 给它几百的百分比
mostly don't need to worry about that just work that as is it's important if 你知道跳出页面多一点吗
just work that as is it's important if 使它更明亮，这是一定的
just work that as is it's important if you're dealing with certain applications 饱和度可以
you're dealing with certain applications 显示不应该这样
you're dealing with certain applications prefer the 8-bit and other times you 如果要重设，则进行采集
prefer the 8-bit and other times you 它将恢复到最小最大
prefer the 8-bit and other times you have very large files and Z stacks and 范围你
have very large files and Z stacks and 因此，如果我们进行转换，请单击重新安置
have very large files and Z stacks and stitch together and it really does help 确保它是自动完成的
stitch together and it really does help 当您在斐济打开它时，您可以
stitch together and it really does help to make them smaller convert them down 仔细检查每个通道并重置
to make them smaller convert them down 只是为了确保每个频道
to make them smaller convert them down to 8-bit especially when you bring them 整个数据范围而不是
to 8-bit especially when you bring them 可用数据，但数据在
to 8-bit especially when you bring them into the 3d software like in Mars and 图片，然后它将转换为
into the 3d software like in Mars and 将该数据放入0 2至5 5窗口
into the 3d software like in Mars and things like that 这将用于8位，所以它
things like that 充分利用了它的宽度
things like that it can help the factor of two will 可用，如果您要键入调整
it can help the factor of two will 并更改为8位，然后更改为8位，
it can help the factor of two will really make a difference especially once 看起来很像触手，那就是
really make a difference especially once 我们想要什么，然后您要
really make a difference especially once you go to three dimensions so 在和之间具有非常相似的直方图
you go to three dimensions so 现在较低的范围稍微更粗糙
you go to three dimensions so essentially all you do is you go to type 整数或的逐步范围
essentially all you do is you go to type 任何结构的强度，但
essentially all you do is you go to type and you change it from 16 you click on 更重要的是我们有大
and you change it from 16 you click on 疾病2x大文件因子2可以
and you change it from 16 you click on 8-bit before you do that you have to be 节省很多时间，我会强调让我
8-bit before you do that you have to be 知道您是否有任何疑问或
8-bit before you do that you have to be aware of there one setting in options 您需要任何特定的新YouTube
aware of there one setting in options 我先去做
aware of there one setting in options which is conversions it needs to be