welcome today today's loss seminar
and I'm I'm truly very pleased to introduce our speaker today
Yukiko yamashita doctor she 10+2 PhD
Kyoto University where she worked with the two hear ya nikita stunning cell
cycle regulation
East after completing her PhD
she remained University which took short
extraordinarily productive postdoctoral fellowship in the laboratory
she cita con examining deregulation genome stability and D
apparent are you curious after obtaining his basic training
regulations cell cycle doctor yamashita went on to do a postdoctoral fellowship
in the laboratory makes
alert Stanford University and it's it's Stanford the doctor machine initiate
studies on the regulation of stem cells using just mill germ-line as a model
system
in 2007 she accepted a position
as an assistant professor at the University of Michigan at the center for
stem cell biology
at the University of Michigan doctor yamashita has explored
how the canonical my tonsils is specifically modified during asymmetric
stem cell divisions
and why modifications are important to stem cell me
more recently she has made numerous seminal contributions
to understanding of the role of central position during asymmetric stem cell
divisions
and how this process central zone and spindle positioning speculating during
aging
stem cells doctor she is a recipient of the 2008 serial Scholar Award
in 2011 she was chosen as a macarthur and 2013
she was named a Howard Hughes Medical Institute investigator
currently docked she associate professor University Michigan
and today we're very excited to hear about her work
in a talk entitled asymmetric stem cell divisions to stop
introductions I'm very excited to be here
so his feelings
a sin X Jan so
I S all said month lol
you buy to sell and then that
you know how so much not Halloween making
bathe a process called cell however a synod
from your division century share
most of the process of making copy themselves buddy at this thing
home you know adapting
kinda a lil different the fate
characteristics so
a few remember no you don't my way while
days but you know where you came to the existence through this wide
each of us used to be a single cell today
we I you know yet you know we are functioning human beings
thinking and talking this on a song
net so instead of being unit 102 something
we are functioning human beings
so why you know hardest come you know where to
he made a big im
processes that starting cell gaw
just simple you know if Insperity
making different mining
same division how now we are made all
cells or function
function as a human being instead of just
back just so that's one thing you know that
you know
this miss any XM division something me
Symantec said
has been Solon idea is
also says me recall I'm
you know the drill to do cells division in a different in
that's why only micro-environment
single now that's like different
and in as a case severs
prepare the region only one
cell some Friday
take different cell
my homework you use stem cell
as a system because the universe themselves
stem cell is the one population he said
cell division so went about us by
discrete to stay as a stem-cell
differentiated me of
showing very
you know special sound like you know huge
3cm song so then of course as you can easy you don't want to keep
of so if you go either side
too many you know the too many them so instead of differentiating so that can
lead to injury can stop
on just on too many don't systems a division
take on the differentiation of it now you deep recast himself
so that you can view sure any longer
leading to tissue
you disease or tissue aging so how do you maintain this balance mine
very nice way of achieving it asymmetric stem cell division to create
one stem cell and the one before shaking so in this manner you stand out was one
stem cell
end up with a month themselves so that means you know there's no net increase
or decrease
customs number at the same time you get this you know one
and i felt that you know doctor differentiated
the plane issue at issue so
we study this question using just saw
yeah measurements them so that it does test this as a murder suspect
so the reason we use the system is
it simply stay not necessary easier these days anymore because you know just
so much technique is available in now
you know in many you know how you can you know how organisms
however still united us your system provides the check excedrin
you know very very you know the per side
you know description about everything so that you can really use stick to
very very rigorous logic in you know
understanding or so in a biological processes
so I love this fly you can
take up test this Justin amado
20 seconds or been there get every
used to it and then this flight this
is very nicely ranked the No you know or so
Fisher arranging a very nice way in a spacious temporary
United or mana so cuz call you to brush structure
from a pic are dependent to go through to pacesetter sigh
if you take a look at the barre tip this is aware
most and the fresh cells which is themselves to resign
net once so does Dubai leave DC
AP got a tip Serres you know to keep
differentiate into a lot more time or the differentiated aside and
around here is a meiotic cells and their
you know after why state become you know would be differentiated
spam orders as you know the bundle looking structure or sounds over here
you know on most mature spock and then if you take a look at the barre
if you got a tip from a test over here
mean and then take a look away you know under the microscope
after staining with various combinations antibodies you can
identify order tied in this tissue in the stem cell niche
federal reserve your son richie is the
accuracy and precision live to study and
to study you know the buyer is using this system so over here showing blue
is called home sells that functions and Michael environment force them so on
needs
so these come cells and across top
might Excel so you know these blue things I
you know just remember my guy so you can probably appreciate their own
10 how cells are making crossed over here
K and then saudis
green cells attaching to the home sells saudis
germline stem cell so in this anatomical context
cells sickly sickening lying on
to the neighboring Germany stem cell so this is
idea house arrest him so is maintained
so because single now essential for this time said identities only provided by
have you have to be patch my crime
or the stem cell niche over here to be stem cell so with that said
upon every division do you them so did asymmetry agreed to create
wants them Serrano differentiating now so
differentiating so it's always geometry
displaced away from cells that's right the cells cannot get any
University from have any more of that Friday commit to the differentiation so
with this destruction Donegal geometric precision
you can other not things with you know incredible accuracy
so and so
way to go I'm during my pasta days right foster
iPhone otherwise so how
do them so the body symmetry that word
you know turn out to be because I'm already ended
division so this stammering stem cell or tea and beat my dick
spin now always perpendicular the exiles
cells so in this manner the donor so it's going to be
always away from this is
you know this is how are you are achieve asymmetric
out to come them and then
intersting re this odeon tation
over the division it's a prepaid way
idea during the same cycle by the positioning of the center's
to measure my good so
what is to create a car that you're single to center song before the PK shah
idea in a cell cycle its always on the side
very very costly and the duplicate there
and after that only once in Tucson migrate opposite side away I
Jeter center some stayed very close have so
even before says enter my toes these
there is no exactly which radio going to do I ended
have so that one don't it's going to be dispatched right
so and then disc I know
unit a menace can be on me down only if you can identified a stem-cell
a single solution by pointing out that this is a stem-cell
as I you won't be able to tell like I unit is
and
it am NOT this center some stays using
them said or their mother center stone
and then jus ad you know the younger daughter center some
if that good differentiated
it not this was the first example
all day asymmetric centrist behavior in any development comtex
after rise time now many
them including you know the human embryonic stem cell you know
neural stem cell I'm appears to
take on a very similar you know you know processed anyway
military suggesting Mac what you find injure Sakura
is a highly likely to become in you know day
you know hi owns so I think I read
like to emphasize no you can start out with a simple
assist them to a lot about ourselves
sock-b_ so I think
fad this united Germany stem cell reside and
niche and then
this meet is supposed to be making stops in nineteen
so follow these stem cells to divide very very fast the point ace
this needs to go has to be demented enough
range so that only stem cell can be exposed to dis
cigna but did not the differentiating now and
in this you know the context of this you know just answering
in nineteen stem cell only in my pics naw
to divide asymmetrically by displacing 1.0
away from are from vintage their
I so these two things are you need a major topic all
might talk today so the faster than me toca body
how oriented set a Division this division plane
VAT you guys and or established
so for you know time people have been getting a maybe
you know that this niche my to be regulating Spain
intention to that works very very simple idea because you know just a minute
the timing systems identity and the ampato
them identities odeon get a division then
the new now also regular stem cell Division play
artificially intelligent that's a very simple question to ask whether
very hard to address why because if you get rid of the himself next inning or
stem cell
niche what happens stem cell is gone so there's no stem cell
left the to score the orientation so that's a very very hard question to
address
so down is the question the past ok we might have a video chat
at tackle so again I'm showing the cartoon by Java John Rice stem cell
attaching to the house hours where germans themselves always divide
asymmetrically by
orienting it's been no and differentiate dota
call gone yet about us is always this press away from the German stem cell
so they it to be known that this have a start
Creek to like guns know why
a semi gone cited by Mike light and are
binds to the you decide to render septa home
or don't miss activate attacks that a pathway
an effect on a path to rein pol for customs and identity
in this system is BMP signaling pathway thats DBP and then
to give leadership so and then when
thing ready and noticed honey oh my that
again your so
this is probably a so if you look at
you know the you PD you know in have a cell
you can see you know day you know they exist at the pump
we think this is a piano seclusion basic and there already is Greece's
package amitai stem cell expressing to bring
GFP so and their one thing of Indian noticed a very
you note the i do. my that this
you PT punk appears to be always very very close to the spindle pole
who just being a part like a and in these K
and Nikki to have a shows appears to compare about operation one punk band so
this is actually the major source
or you know the one pound packed day is a major source of this and I'm
mine and in each have a cell so its maybe not too surprising i mean this is
only
how to say um so this you be deep and bank south or
souls over the UP d light on
for this single Gemini Sims and then
so video was very interested in what's gonna happen if you get little single
pump
so that's what she exactly did so she could deliver
operation experiment just removal one Ponca
K so when should be dad and then if you use you watch is away
we did to wats under this neighboring John Rice them so into my justice
and then what you see is a spin there is no Patterdale
to himself it's not particularly oriented anymore
and then
so if she's school I so it's
very very clear that in a normal situation spenders a hundred percent
already ended
however once you populated Hispanic that and then to take a look at the spindle
orientation
mohabbat vendors are not correctly oriented
and then most critically a
why expand my the country expanded a very much like
is back whenever you operate a single upto right
the US simply tweeting hoping this generous them said right next to it
and Mr Justice however of a case of course
it's like a fishing you public here wait wait wait and their
maybe some generous them so far away from
American a punk that in time I does and then you can trace court case that
over here so in this case
no artists been no I'm oriented at all
this suggest back this u-pb
pumped out what this morning cure its beauty debut dating
spin to rate it orientation right next to it
instead of far away so this kinda suggest that it's not just about
you know the
a me tonight on is secreted and if you stand in to control
everything about the German I stem cell 2d Tommy
including are you know added
you know spindle orientation so and then
but of course you know the fair you know you know doubt
a crush on about it this experiment is that standard issue PD's a very
essential factor to maintain this time said identity
so if you get little bit right so systems that are simply stop being stem
cell that's why they can just audience at all
that's one possibility so next or what a period it was just to take a look at
or you know pathway components or
this you know the Jackson Hathaway to see if they
regulate the spindle orientation why not so she take a look at the U P Diddy
sizzle I gotta chablis
to buy it is operation and now she also did
RNAi to be moved bases from in I have cells
even jaded 32 the sims in Los you can catch the right timing
where I'm in stem cells are not completed
lost about us you can see you know the effect on the specs been there are any
indication
and if you look down UPDA you can see very very high frequency spin on
messenger summit orientation
i cant and if you take a look at the next you know
connects never downing a pasta racer accept outside a crime
right bicep sector going to step down like a step that dominates
doubt also cause a very high precancerous been
and Asian but billion importantly
Jack kindness and a stacked it doesn't really cause any spankings
orientation at all here and then
these conditions because you're knocking down Jack Spade
pathway you know
final reno is gone
these conditions been charities to the stem loss
suggesting that indeed stems all or something identities compromise over
here however
spindle orientation starting fine so this is really suggest that the
functional
or night and another set it's probably
independent also transcription minute walk a part of you know this time said
identity
so back to how we got really interested okay maybe we should take a look at
these two more gears tonight on an effect that
if team you know I'm how they may be regulating the spindle orientation
so the first thing on you know the next thing you know a period it was to take a
look up to
Domino you know that a ship that organization so in
cafes as you expect artists to step down local ties to the right place
where to stem cell attached to this I'm sending it to ourselves over here
okay so costas has that effect all icon
that's coming from the have receptor should be organizing around here right
so then but however I these centres
start approach into my toes is two things happen number one
two days you know that affect us bad accumulating
on the spin the report and then in the metaphase by mid FA's when centers
you know just themselves upon fully you know the phone
spindle this this setback is on the spin
K so that was something really I expected and of course we wanted to know
this localization
depend
like so I'm activities
so in interphase you see you know you barely see any
you know the concentration all the Domus
septa if you get laid off like K
and then by face
date right to localized on the spinner Paul
but then you know by metaphase
that you know this spinner organization that except I is very very diminished
and what thing I want you to notice is fears so
this is not just the usual hope and pray issue
its always that approx multiple that every Friday know many you see that i
dont coming
that everyone that's mostly affected
you know remote affected by this you know that raio
like on did accept the capital guys to
the proximal spin report but dispense with her poor seems to be doing to be
okay
and then you can't gaw and that you know the major the ratio
or this don't Minister organization on approx monopole
process this terrible in the water type situation yes they
localized just one to one ratio however
now in upon knock down the line and
know what you see is the ratio goes be told
you know one suggesting that an approximate call
localization is significant animosity
affected by this you know to me be moving
the move at all make a K
so I think this is just the control so
me I'm honest should so then
re we've nearly 1.8 you know what what
receptors doing ok arising on
Espino and then part regulating this
you know spindle orientation so we thought there should be some
you know there's some can in their introduction to something
that you know that could explain to see not too
its function on the spend on NASCAR vision that we take a look we took a
look at this in a just
interactive database dat has actually been very high quality
precipitation spectator and no when we took that peers
looking for that Daw Aung septa you know contract out what are we found with
edye that microtubule binding protein
you know percent and I could you buy me a broad and quite a haul
many aspect my goto dynamics and I we confined to my in the press
Bay shown that Daum nice and their
a you know that you are his goatee interact
and then if you knock down bb1
now you can see that that also cause a high precancerous been there miss
orientation
Germany stem cell that how that happen and then we
looked at you know sequence of the just don't miss the fact that
a little more carefully and what we found is dis
sq I P sq XP is a powerful much
you know that's me already figured out %um binding motif
so ok Austria in the process of making this mutant
and to see if this is required of all he be on binding
i holed spend orientation so
no cost dream outcome is this sq
I P Diddy shown on me compromise my good to go
instruction on also on me spindle orientation
functional we've not be moving you know the Jackson a pathway activity
setback that kinda mutation is going to be a separation
function mutation so that we can go to structural tubing
so that's something you know we are very much looking forward
right now then a so
E division ship it to you on a DOM so
and as expected you know the EDU I'm
localize to spend the ripple or and spend a greater
their up on Dom on any I you know once you give it up this
you know need to receptor 81 can't organize
on the spring there anymore K and their again if you take a look at the Ferry Rd
phase around here
again I think you know you might notice this
in a proxima side all that you do an organization on it's been reported
the map and if you quantify again you can confirm this
pic 81 organization is much much Rica
on the on approx monopole up on knocking down
all receptor dimers so I'm
is in a AB on most specifically approach to my spinner Paul
as a writer Domus you know dairy
ago dos you know the specific I specific
loss over Domus on the proximal spin the ball
upon knockdown I this view suggested fact
so via anything special about this property: my poor
that is I'm I'm coming or attached who perhaps inside
so the Mico tumor may be under a different on the fact that was the idea
so that means you know the UPTU domicile EB
axis might be regulating something
on this I today so the idea is maybe
you know that I can is coming here and their on
this specifically on this side Domus intercept I somehow helping to maintain
or like you to rate this special microchip dynamics
specifically on this time so that's why if you give you know these things
and that you know the microtubule dynamics over here messed up
I'm and eating spin them patient idea
so the based on this idea that microtubule dynamics might be different
on this I compared to the distaff side
we didn't mind experiment riches you know
just checking the microtubule dynamics by for compatible
to me so I'm gonna play a movie just in a sec on about to re-enter
sorry this is actually upside down in the between two things
so this you know asterisk always shows how to sell so
in this spend all this is Mrs them sending the spindle proxmire poorest
facing up and then in this a cap move be
having facing downside proxmire sided
facing down I'm sorry about that and in this experiment what
what a period it lies for the combined on that haha or
you know the part of the props Marple orchestra Paul
so that I movies going to shoulder for to come by
for the combustion of this jury only at the proximal
the law is opposite side so let me show you the
be so if you Khambhat
the props MARPOL over here registers up over here
and a very quickly this Michael to go is over job
to the opposite side as well suggesting that a micro tour is very very dynamic
over here so that it's gonna come to genocide
very very quickly me show you one more time and then meantime
let me also pray jazz a movie here
your combating distance I this microtubule
BRT make it to you I know no matter how long you a
so women tried
to status maybe
at the same time so they're probably hoping you can see the difference
its berry Creek from here to there no proxmire to this now
but they're it's very very distraught to for them I could do a two-man
from this to
proxmire side so correct every
what we think is so the proxmire poor
it's different to probably I'm gone
audience Michael to go you have to have produced throng of cost
to say I'm got because of the strong of course you have to have you know the
stronger
I'm going for he's been forced over here
which is probably regulated by tonight on and receptor
so and then this is a way that we think you need to stabilize the spindle
in order and get away idolize you know how to suspend could be spinning
so but despite a poor a sick I know
I had a very very stable poor so one thing probably I can meet you know
one analogy I can make it back you know
and I'm shaking with you and then
week false right back to regard mister Paul
and then nothing is really and dynamic so you just smile and shake
decide may be stable
but they're the you know me I know you are seeking ways you know as
you not there there you know with all our might like
very very strong shape and as long as you are you are false and your power on
my power as much a
it doesn't look at very different from the other side
however because actual forces very very strong from both sides simply being much
if one-sided a say you know you know
I weaken my foster back you I'm shaking forces going to overwhelm me and now I'm
gonna
started spinning saw that that idea get
so a
and their yes so they probably days the unique for some spin dynamics to
on investment no in the germline stem cell message
and then second thing is this need signaling
is really directory regulating or
you note the arm this unique spin there for storing data
I was maybe I should say the needs
spin know you know that niche signaling is really potholed
holding to spin the report to the right place okay
so let me see the Kia a little bit I've been telling you that okay
needs C-nine is kinda very limited so that
on me germline stem cell can
X can be exposed to this and singing over here
break how and then in the only in this context spend their orientation make
sense
because only ending spend oh it's going to take away one daughter
out the dissident range however however imagined
how need says know exactly how much
light on it to produce so that this kind of a minor man is created
being analyzed and now you know the imaginary experiment if
this have house awesome 7/8 expressed so much
or that my god great and then
so no matter how you nicely audience to spend all
stereo boss daughter is going to be under in France
or facing now so the question has been okay how
you note the Ranger
you 7 a.m. severely physically limited is that only just simple secreto so that
you know those things be sure it to you today to buy time you know the things
come to their
differentiating daughter is that you know heart is a house sells out
okay
we've just hoping matter you know what they are making is
just enough not too much not too little so
we thought that that kinda a little sloppy
for such a you know the beautiful system 2d be precisely rocking
and then we I think you know we got at least a poder I said to it
so this was single Hyundai done by a very very time to possibly
you know about so for the possum she had to be located
UT Southwestern and their so that's how I couldn't for
you know moving house all week set up the car ratio is Michael Bouchard
whose accredited member beauty so stressed out
buckingham being examined himself so I asked him to be a local host
for my you so that we can keep doing this saying what but then now in a
corporation with Mike as well so
Monday so my it came to me saying that generous them so has a very
cue funny preclusion into himself
so in this micrograph is showing a
you know the two men Jie p expressed
only know John Rice themselves so the germans themselves surrounding
you know about eight of them right now over here is facing toward home sellers
which is that because it's not
you know the stems as I doesn't expect to be in GFP
them marked by asterisks so my is over the vision was that
this all genres them some also the generous terms and I have nice
protrusion
always into themselves so there
the my first reaction lies you know my you
Kamal so we have be using to BGP forever
you know and i DK if not centuries so how come everyone has missed she said
this seems to be refreshed structure if you fix it
mostly use it and you have to probably take a look at the lame sound just for
you using
GP
without fixing that the best way to look at this
so with how guys instead of doing a new experiment to confirm this I just simply
went about me
very very order for a doubt be my very own a computer
buckingham my pasta today's and then
I just that go they may ish and then there you go haha
so here added one John Rice stem cell
the poor game this nice pretty going to have yes I was so that it could be a
stand now the st.
at that time I was busy looking up to spend over here that's right mysticism
K so and then so we wanted to know a little mall a baller destruction because
you know where you say
protrusion hawking into the house what does that mean
is there any Horry or you know what kinda but dr. John mitting
structure so we did daz3d
constitutional the structures are in this particular image
this is your main stem cell and now I'm so this is a problem
Jean going into the half and then this red is the mocca
mister donohue John Makar between
generous them so I'm have a seller's and these
add to you know the same cell junction between to have felt
pic to send this I'm gonna rock at baseline and you can probably see that
are this sadness or this
prostitution doesn't have to set out a huge amount to get over here
rate and in a second
this thread is going to disappear so that you can really see the protrusions
there
K over there so now you see the book
but then have said is a VAT program
you know the facts the house at a broken to accept this you know producer John
which isn't that I'm gonna shower scene if your second
I but for a few reasons we decided to call it is my go to replace an attitude
because of the structure similarity that be identified
just to his name coming managers
and then the reason why we knew this is not the primary cilia
walk acting basis structure called just like me
wean renewed this is ease up these
and then turning them into have the broadest you know the finish on
in terms of structure and immature competition so we Faridabad
fair to name it them manage it but then
to make sure that this is a microchip better structure we decided to call it a
microchip
narrative or in 15 mg so then
this is showing Mac are actually it is not puncture
on the seven-member so if you take a look at the micro to remember to go over
here
poking into the house as you see the membrane surrounding it
and then this you know the powerful are ace member and I
so you can see you know this not to be surrounded by to present a membrane
again let me show you the movie quickly
so and
you from the dockside you see this number two
and said this paper is going to go away
so that you can see inside is your
inside you know the narrative is parking and and their
five or more rock we know this is that this
profits amounted double to present a member that means
generous them since I have a membrane
on now a on the fact is a manager
at the same time have a suicide also invest in a tux at
this you know narrative because you can see
by using this you know mistreated you be specifically expressed
in a John my same cell in the house their side
Boise game you note the even mean eight
this might go to imagine suggesting not at this office oven and 2 B's
to present a member long coming from the German stem cell jus are coming from
absence I
said a
this narrative is very very specific to the stem cell so
almost every single generous them so hard manager
but it's very very very very rare in a differentiated
germ cell and their in the Chumash them so it always
facing toward ourselves
Savannah also I saddest microchip narrative is not the primary cilia
want a refund is our so this
dis magritte even at a restaurant so Dissidia components and I
you know the into fresh in a transporter proteins or
you know the city anymore down for example corrupting air is no crazing to
a microchip
manager new or as I you know that i ft components are also getting to my car to
narrative so and this is kind of nice because not just
I'm you know does have a shot at least 3 seed America competition
them by looking at these you know the competition with yasser
test requirement and their if you take a look at also see you know
com you know that we take a look at the pretty much everything and then there's
a few
affect on an actual earnings or thickness
so why its University remote a that is known to suppress
Cydia growth a debt if you knock it down very similarly
microtubule narrative also gets much much pick up
and their if you're not now i ft complex
you know the component that you know horses I the friend intro fresh air
transport proteins
now you whose links over the manager so this is good
function made it home so and then
so of course we wanted to know what the kinda function this is really you know
this is my good to go in an interview having
and then you know them I knew when I headed to take a look at
also seen a possible sedan combines
you know known to be quite awful stem cell
you know I'm sorry you know the instruction so
I'm I
you mentioned idea one pathway to UB
rate you P thx pathway Jenna with BMP
you know the pathway know when I was looking at
or everything and my you quickly DNS you BB
or unit attacks at a component seem to have nothing to do migrated
manage it instead what issue fun is
his BMP pathway seems to be involved in
you know or their functions
through the micro chip banana chips so this is a
have control every few expressing GFP
using jammer specific driver you can i sorry
illuminated demonize them said over here rate because of course you are
expressing GFP
in a stem-cell you should be able two systems over here and then this
area which is Brock out this is the home sells
K however instead a GFP
when she expressed you know DB press to respect our
which is called TKB Big Bang in Sophia
now this is still expressing the same place
as JP possible here all or
TK BJP was ob up in himself instead of germans
so that means this to give each if you still must be made around here
but or moving into themselves so
why this is happening so this is something I do you like about yourself
or you can make all those
baking a background you know
completed a dark I'm everything so so that you can really get them very very
nice research on the set a virus
so in this particular experiment Miami made you know the single Kwame
singers armrest them say expressing to be in GP
and their this Sep 30 Kb now you can see the single was your man stem cell
its shooting very nice
you know the microtubule nurturing to have a7 here right
and then this exactly the same cell is also expressing
red you know MJD have PKB
now you can see this image 80 Kb is
on them I could do it so this is really suggest
indeed it is receptor all good B&B
you know pass right is generated in necessary body over this German stem
cell
and then traffic to the microchip narrative K
Minnesota you know this is how we think you know the German stem cell shootout
this might go to another job
90 Kb receptors made inside a generous terms are moving into the office hours
along the microchip K and then
so is is this a where I unity Kb
buying tonight and
gets activated it seems so so what we see is now in this experiment
DPP GFP you know tonight I'm
GFP is expressed %uh from the home side and then
distinctively jerry is expected from the German side
and then they completed colocalize in nablus out
so that means this you know the TKB to step back
is traffic to that microtubule managed to
that's where in a bind to it tonight and that's coming from the house out
so that means instead of
how the service greeting the DPP to the outside water so that a generous them
expected to look for it and get it and spare
this like you know the stem cell niche like I'm not accept instructions are
happening
specifically at the socket my could you imagine K
met we even added you know addition experiment this is a core expresso all
just a regular to give me and Jerry I just tip
Lisa G Panama that becomes JK positive
on iraq it should right unabomb these also call okay completely
so this suggests that not just PKB protein
Sep game but it acting befall that's located
on and I wanted and then so
because we wanted to make a connection between just observation broker this
observation on them function are to come
so one thing is you know meant as I said
you know because we know there were two genes are organizing on the microchip in
an interview can manipulate
rate and their what we see is I
if you increased my go to remember to you see more over the TKB
in have a Dr compared to the poncho the state if you give me love them I could
do bernard
what happens is TKB instead of going into the house sells over here
is bad organizing on a problem
with the germans themselves so that means this you know i ft component
special legally required to traffic 50 Kb
made in the germline stem cell into a
you know microchip managing K and their
because we wanted to take a look at the readout that Acer
United BMP pathway activation
which can be monitored by force for mining so my mind that the force was
smart apiece much then
this is a writer type situation where germans them said hi
some you know basic you know based on mondo
United DVB activation on the numbers themselves
f you increase you know that pic
manager what happened is to say that I get to where you
and if you get rid of them I go to burn them into
listening to get to where a week net occasions over here
so yeah if the more my could you imagine
Maw dvb-c many and then left my coat your natural less migrant
I'm this debasing nazar
about because if PPP passerby in the pathways required
systems maintenance which has been already known for some time
so if you get me dog microchip unanswered by setting mutation over here
and over here now what you see is a program
miss them maintain so compared to wanna take control right which maintains a
stem cell number directory
okay so these mutant cannot maintain a stem-cell
rap so that you know I think
so
I I wanted to summarize what they told you today
so the fast ISO you know this later part of what I torturous
so we identified at this previously unrecognized structure which renamed my
go to banana chips and then
this microchip now don't you penetrate
in to customs needs and then in this way
you know only stem cell can
engage in a productive you know they need
stem cell you know you know the singing
so in this right now you know you have to woody
how much I have a sense have to produce laid on it because it might be fused too
far
because it probably doesn't defuse at all K
so then so we think this is probably you know the functioning as a mechanism
the baby said active non diffusible stem cell need
sickening and idea part of my talk I also told you
you know needs singing that has outperformed
sedan which is you know the Jackson to pass rate you PTI Domus
the sector is right now
it doesn't want to know much about all we take a look at that
and their it doesn't go to the United manager but then stay on the south yes
where they also and got to spend oh so that it they
always divide you know resorting to spend no
so that they can always be made a symmetric so
no 48 on time I was always very very curious why
you know this I'm sorry to have to see pathways
now I fear that you know we started 78 in these
to you know the you know then it's right on the units in
pathways so you know why is really doing a very very
burning you know the functional general on
pro you know there may also you know generalizability
regulating spindle orientation berry you know in
you know 188 an amana on top of that my
my guess this from here that my guess is
because this you PD doesn't utilizes manager
I suspect that this issue might have actually diffusing
so then in that case so if anything happens to
germline stem cell ban you have to probably you know
to this a different shipping set out to because I them back
now to function as a stem-cell now
you can use just second the same to for example you know lead
either differentiate so that you can pick up by the jammers stem cell
and their so by having this you or suspend 1
you know in net presents or function stem cell
no I just hours can be a complete to come to the stand
Nate however anything emergency happens
right and then out just once they were nice enough to begun by the situation so
that way
you can maintain the baby stem cell for as much as they are really there
and as long as they are there however eyes it's gone you
you have a plan B sought to maintain a stem cell
you know on the system's appropriations and they're so that you can maintain
their
this shamanists bassist
possible so we stacked I'll care so
to spend their orientation upto Potter standby
of India Chan very very dedicated past ok
and so did not to a party was down by marina
as a very very dedicated pasta she's ready for
job any right now star if you're hiring
she's fantastic I'm saying back everywhere
I think you know she's just I always think myself like
I can't I'm fine Maw she's only person who makes me think all here is a partner
who might love
even more than I do some I think you know she
I say shifty because
you know she's gonna they view the high our debate by walking along I'm
honor so and then she has
only meant that it's also the imagination in a very very productive
imagination
she's gonna d a great you know be a great party member
sup that's it man happy to take any questions
thank you very much for your answer
hired two questions about your Nana to
it's the first day is do you see exchange with the cytoplasm with insider
the nanotubes
knows a primary cilia don't exchange freely do the same thing and also to
between a damn right themselves that's great yeah think
the diffusion yeah I week don't have
or a is family morning cue
never like GFP
if you just make just GAAP
in evolve in I'd
I think base there should be some active maker
that you know that let them go into the migrant
so I ABT about it
the data is not the strongest yeah but I think there should be
and see other question is more broad you see these in any other circumstances
supplies don't have primers silly and they're so much signaling that takes
place on primary cilia
is this their substitute yep that's very very
you know great speculation II
think that might be yeah I that that deflected away
you know we are now going so did
arrest or you know because as a status
you know prejudiced fixation I don't think anybody created
now you just simply every you know everywhere
and then this might be you yeah great idea
me feel free to looking to this year
up
already so we so far looked at the Phoenician I'm stem cell
isomers somewhat fixed themselves in in a also them seems to have this Michael
but we haven't
characterized sufficient to be able to compete and they say
how different day our their function at all
we don't know about it there exist 92 like sharks
in Alyssa to either
house statement microtubule
managed to capture good question yeah is it changing with time
to be looks like an anchor so I'll the run II
decided not to include to my job because that was too much
so that their behaviour is kinda very similar to
primary cilia that means interface they maintain my go to United baby's favorite
if you do the movie it doesn't change lens sawyer
bigness at all whole our eyes but right before my
to assess it gets the truck into
you know that way its retracted and then right after my toe said it comes back
just like primary here but it's not the primary cilia
that's very funny behavior so in the end up a bit very stable but
doing said it's likely comes out there you know disappear comes out this idea
so this is a pretty unique system be
and online stems
I don't think so because for example ok
something very similar maker 202 it's called the site me
that acting basic structure about you know if you look at the gym
Jean boxing and new fast this item
bombing address security issue to maybe a
you know a few seem like you know the DPP as
you know if yes you know this again I
and then this very similar site me
now on in you know that you can we r a few hours are you not about Africa
systems to
so by and I'm you know I would be surprised if the finding a microchip
narrative in them in about the right to suspend
but at the same time as a baby fantastic idea is
you know maybe you know them I could you imagine the sub-sector primary cilia
in which case in about every high as a primary CSR maybe they don't need it
so either way that's fantastic thou I mean we
wanna be agreed to that thank you hi I was
a curious about them again is in the koreans banana you so if you would like
to pick the Express
BBB some this girl would you have created yes we did yeah
so we ectopically expressed the view from
completed violates our now damn nice sounds even
even you don't have to be done by stem cell even differentiating spermatogonia
started shooting this manager but what about the Apple yeah thanks
really great talk house sure you
that there's no motor taking the TK
p up the microtubule managed to
really did fusion based or resume
be a motor anymore any reno
I only see you need ruft rating pressure transport but exactly how
TKB is trafficking tonight about how about that
I don't want to comment on it we don't know yet yeah at school
really good sorry in a very basic
much I fully understand so these models that you're doing are they
all in the Drosophila are this is in in vitro
that you see musician actor you know day
you know the tissue right outdoor you know that their software
so some that especially
the shoes sometimes we you know to fix them right away after we exited
should measures of and or sometime a little bit over show X
culture to do you know that I V me right so it actually
do you ever see the cell letter better
not the stem cell anymore they're different shit but the company's them so
they never have this microtubule or
even something like Iranian no medical missions differentiating said
very hot any bit but you know eso
week I Q that 0.007
managers pastel something like that so it's
not there are but a bury any
thinking thank you on that now they would like to think that I yamashita
here and I ate
on great thank you very much I said okay
I don't wanna and I want to remind everybody there is a little reception
right at the library please
join us at the reception to on the premises thank you very much