MALDI TOF过程分为两个阶段。 The MALDI TOF process is a two-phase procedure.
最初，将样品固定在晶体基质中。 Initially, the samples arefixed in a crystalline matrix.
在最初的电离步骤中，它们被激光轰击。 In the initial ionization step they’re bombarded by a laser.
样品分子蒸发到真空中，同时 The sample molecules vaporize into the vacuum while
同时被电离。然后施加高压以加速 being ionized at the same time. High voltage is then applied to accelerate
带电粒子。第二步是飞行时间质谱分析阶段。 the charged particles. The second step is the time-of-flight mass spectrometry phase.
在线性模式下，粒子将撞击到内部的线性检测器上 In the linear mode, particles will impinge upon the linear detector within
电离后几纳秒。更高质量的分子将稍后到达 a few nano seconds after ionization. Higher mass molecules will arrive later
比轻的。 than lighter ones.
飞行时间的测量可以实现 Flight time measurements make it possible to
直接确定分子质量。光谱中的每个峰对应于 determine molecule masses directly. Each peak in the spectrum corresponds to the
从电离矩开始，沿时间轴的特定质量数。 specific mass of the particle along the time axis, starting with the ionization moment.
在反射器模式下，粒子被转向，以便它们飞向 In the reflector mode, the particles are diverted so that they fly towards a
第二个探测器。除延长飞行距离外，反射镜还 second detector. In addition to extending the flight distance, the reflector also
集中群众。这两种效果的结合使更高 focuses the masses. the combination of these two effects makes for higher
这两种效果的组合比线性模式具有更高的分辨率。 The combination of these two effects makes for higher resolution than in the linear mode.